2002 cytokeratin 1 (Proteintech)
Proteintech is a verified supplier 
95
Structured Review
Proteintech
2002 cytokeratin 1
2002 Cytokeratin 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2002+cytokeratin+1/Cytokeratin+1-specific+Antibody/pm34352263-286-196-205
Average 95 stars, based on 48 article reviews
2002 Cytokeratin 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2002+cytokeratin+1/Cytokeratin+1-specific+Antibody/pm34352263-286-196-205
Average 95 stars, based on 48 article reviews
2002 cytokeratin 1 - by Bioz Stars,
2026-10
95/100 stars
Images
Related Articles
Western Blot:Article Title: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. Article Snippet: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation Avinash Padhi, Ankit Srivastava, Abarajitha Ramesh, Marcus Ehrström, Michel Simon, Enikö Sonkoly, Liv Eidsmo, Peter Bergman and Josefin Lysell Increased presence of IL-22þ cells in the skin is a characteristic finding in skin barrier defects, such as psoriasis and atopic dermatitis.. However, mechanistic insight into effects of IL-22 on epidermal functioning is yet to be elucidated.. One crucial step during epidermal differentiation is deimination or citrullination. Article Title: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. Article Snippet: List of Antibodies Used in the Study Antibody Application Working Dilution Source (Catalog Number) Anti-PAD1 (rabbit polyclonal) Western blot analysis 1:400 Abcam (ab181791) Anti-PAD2 (rabbit polyclonal) Western blot analysis, functional blocking assay 1:1,000 Proteintech (12110-1-AP) Anti-PAD3 (rabbit polyclonal) Western blot analysis 1:1,000 Novus (NBP1-92240) Anti-PAD1 (rabbit polyclonal) Immunofluorescence, immunohistochemistry 1:200 Sigma (HPA062294) PAD1 mAb (Clone 6B4) Functional blocking assay 1:1,000 Cayman Chemical (22997) PAD3 mAb (Clone 4E5) Functional blocking assay 1:1,000 Cayman Chemical (24377) Human IL-22 Ra1 antibody (polyclonal goat IgG) Receptor neutralization assay 1 mg/ml R&D Systems (AF2770-SP) Normal goat IgG control (polyclonal goat IgG) Receptor neutralization assay 1 mg/ml R&D Systems (Ab-108-C) Human IL-10Rb antibody (monoclonal mouse IgG1 clone #90220) Receptor neutralization assay 1 mg/ml R&D Systems (MAB874-SP) Mouse IgG1 isotype control (monoclonal mouse IgG1 k clone #11711) Receptor neutralization assay 1 mg/ml R&D Systems (MAB002) Anti-modified citrulline, clone C4 (mmAb) Western blot analysis 1:1,000 Merck Millipore (#MABS487) Anti-peptidyl-citrulline, clone F95 (mmAb) Immunofluorescence 1:200 Merck Millipore (#MABN328) AHF7 (FOVA711C) (mouse monoclonal) Western blot analysis 1:1,000 Simon et al., 1995 AHF3 (FOVA311C) (mouse monoclonal) Western blot analysis 1:1,000 Simon et al., 1995 Anti-deiminated keratin K1 (ACP) (rabbit polyclonal) Western blot analysis 1:1,000 Ishida-Yamamoto et al., 2002 Cytokeratin 1 (rabbit polyclonal) Western blot analysis 1:1,000 Proteintech (16848-1-AP-20) Beta actin mouse mAb Western blot analysis 1:10,000 Proteintech (66009-1-Ig) Goat anti-mouse IgM (heavy chain) antibody, Alexa Fluor 594 Immunofluorescence of F95 10 mg/ml Invitrogen (#A-21044) Goat anti-rabbit IgG (HþL) Alexa Fluor 488 Immunofluorescence of PAD1 1:100 JacksonimmunoResearch (111-545-003) Goat anti-rabbit IgG (HþL) HRP Western blot analysis 1:10,000 GeneTex (GTX213110-01) Mouse anti-IgG antibody (HþL) HRP Western blot analysis 1:10,000 GeneTex (GTX213112-01) Goat anti-rabbit IgG antibody (HþL), biotinylated Immunohistochemistry 1:400 Vector Laboratories (BA-1000- 1.5) Abbreviations: H, healthy control; HRP, horseradish peroxidase; L, lesional; PAD, peptidylarginine deiminase. Functional Assay:Article Title: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. Article Snippet: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation Avinash Padhi, Ankit Srivastava, Abarajitha Ramesh, Marcus Ehrström, Michel Simon, Enikö Sonkoly, Liv Eidsmo, Peter Bergman and Josefin Lysell Increased presence of IL-22þ cells in the skin is a characteristic finding in skin barrier defects, such as psoriasis and atopic dermatitis.. However, mechanistic insight into effects of IL-22 on epidermal functioning is yet to be elucidated.. One crucial step during epidermal differentiation is deimination or citrullination. Article Title: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. Article Snippet: List of Antibodies Used in the Study Antibody Application Working Dilution Source (Catalog Number) Anti-PAD1 (rabbit polyclonal) Western blot analysis 1:400 Abcam (ab181791) Anti-PAD2 (rabbit polyclonal) Western blot analysis, functional blocking assay 1:1,000 Proteintech (12110-1-AP) Anti-PAD3 (rabbit polyclonal) Western blot analysis 1:1,000 Novus (NBP1-92240) Anti-PAD1 (rabbit polyclonal) Immunofluorescence, immunohistochemistry 1:200 Sigma (HPA062294) PAD1 mAb (Clone 6B4) Functional blocking assay 1:1,000 Cayman Chemical (22997) PAD3 mAb (Clone 4E5) Functional blocking assay 1:1,000 Cayman Chemical (24377) Human IL-22 Ra1 antibody (polyclonal goat IgG) Receptor neutralization assay 1 mg/ml R&D Systems (AF2770-SP) Normal goat IgG control (polyclonal goat IgG) Receptor neutralization assay 1 mg/ml R&D Systems (Ab-108-C) Human IL-10Rb antibody (monoclonal mouse IgG1 clone #90220) Receptor neutralization assay 1 mg/ml R&D Systems (MAB874-SP) Mouse IgG1 isotype control (monoclonal mouse IgG1 k clone #11711) Receptor neutralization assay 1 mg/ml R&D Systems (MAB002) Anti-modified citrulline, clone C4 (mmAb) Western blot analysis 1:1,000 Merck Millipore (#MABS487) Anti-peptidyl-citrulline, clone F95 (mmAb) Immunofluorescence 1:200 Merck Millipore (#MABN328) AHF7 (FOVA711C) (mouse monoclonal) Western blot analysis 1:1,000 Simon et al., 1995 AHF3 (FOVA311C) (mouse monoclonal) Western blot analysis 1:1,000 Simon et al., 1995 Anti-deiminated keratin K1 (ACP) (rabbit polyclonal) Western blot analysis 1:1,000 Ishida-Yamamoto et al., 2002 Cytokeratin 1 (rabbit polyclonal) Western blot analysis 1:1,000 Proteintech (16848-1-AP-20) Beta actin mouse mAb Western blot analysis 1:10,000 Proteintech (66009-1-Ig) Goat anti-mouse IgM (heavy chain) antibody, Alexa Fluor 594 Immunofluorescence of F95 10 mg/ml Invitrogen (#A-21044) Goat anti-rabbit IgG (HþL) Alexa Fluor 488 Immunofluorescence of PAD1 1:100 JacksonimmunoResearch (111-545-003) Goat anti-rabbit IgG (HþL) HRP Western blot analysis 1:10,000 GeneTex (GTX213110-01) Mouse anti-IgG antibody (HþL) HRP Western blot analysis 1:10,000 GeneTex (GTX213112-01) Goat anti-rabbit IgG antibody (HþL), biotinylated Immunohistochemistry 1:400 Vector Laboratories (BA-1000- 1.5) Abbreviations: H, healthy control; HRP, horseradish peroxidase; L, lesional; PAD, peptidylarginine deiminase. Blocking Assay:Article Title: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. Article Snippet: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation Avinash Padhi, Ankit Srivastava, Abarajitha Ramesh, Marcus Ehrström, Michel Simon, Enikö Sonkoly, Liv Eidsmo, Peter Bergman and Josefin Lysell Increased presence of IL-22þ cells in the skin is a characteristic finding in skin barrier defects, such as psoriasis and atopic dermatitis.. However, mechanistic insight into effects of IL-22 on epidermal functioning is yet to be elucidated.. One crucial step during epidermal differentiation is deimination or citrullination. Article Title: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. Article Snippet: List of Antibodies Used in the Study Antibody Application Working Dilution Source (Catalog Number) Anti-PAD1 (rabbit polyclonal) Western blot analysis 1:400 Abcam (ab181791) Anti-PAD2 (rabbit polyclonal) Western blot analysis, functional blocking assay 1:1,000 Proteintech (12110-1-AP) Anti-PAD3 (rabbit polyclonal) Western blot analysis 1:1,000 Novus (NBP1-92240) Anti-PAD1 (rabbit polyclonal) Immunofluorescence, immunohistochemistry 1:200 Sigma (HPA062294) PAD1 mAb (Clone 6B4) Functional blocking assay 1:1,000 Cayman Chemical (22997) PAD3 mAb (Clone 4E5) Functional blocking assay 1:1,000 Cayman Chemical (24377) Human IL-22 Ra1 antibody (polyclonal goat IgG) Receptor neutralization assay 1 mg/ml R&D Systems (AF2770-SP) Normal goat IgG control (polyclonal goat IgG) Receptor neutralization assay 1 mg/ml R&D Systems (Ab-108-C) Human IL-10Rb antibody (monoclonal mouse IgG1 clone #90220) Receptor neutralization assay 1 mg/ml R&D Systems (MAB874-SP) Mouse IgG1 isotype control (monoclonal mouse IgG1 k clone #11711) Receptor neutralization assay 1 mg/ml R&D Systems (MAB002) Anti-modified citrulline, clone C4 (mmAb) Western blot analysis 1:1,000 Merck Millipore (#MABS487) Anti-peptidyl-citrulline, clone F95 (mmAb) Immunofluorescence 1:200 Merck Millipore (#MABN328) AHF7 (FOVA711C) (mouse monoclonal) Western blot analysis 1:1,000 Simon et al., 1995 AHF3 (FOVA311C) (mouse monoclonal) Western blot analysis 1:1,000 Simon et al., 1995 Anti-deiminated keratin K1 (ACP) (rabbit polyclonal) Western blot analysis 1:1,000 Ishida-Yamamoto et al., 2002 Cytokeratin 1 (rabbit polyclonal) Western blot analysis 1:1,000 Proteintech (16848-1-AP-20) Beta actin mouse mAb Western blot analysis 1:10,000 Proteintech (66009-1-Ig) Goat anti-mouse IgM (heavy chain) antibody, Alexa Fluor 594 Immunofluorescence of F95 10 mg/ml Invitrogen (#A-21044) Goat anti-rabbit IgG (HþL) Alexa Fluor 488 Immunofluorescence of PAD1 1:100 JacksonimmunoResearch (111-545-003) Goat anti-rabbit IgG (HþL) HRP Western blot analysis 1:10,000 GeneTex (GTX213110-01) Mouse anti-IgG antibody (HþL) HRP Western blot analysis 1:10,000 GeneTex (GTX213112-01) Goat anti-rabbit IgG antibody (HþL), biotinylated Immunohistochemistry 1:400 Vector Laboratories (BA-1000- 1.5) Abbreviations: H, healthy control; HRP, horseradish peroxidase; L, lesional; PAD, peptidylarginine deiminase. Immunofluorescence:Article Title: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. Article Snippet: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation Avinash Padhi, Ankit Srivastava, Abarajitha Ramesh, Marcus Ehrström, Michel Simon, Enikö Sonkoly, Liv Eidsmo, Peter Bergman and Josefin Lysell Increased presence of IL-22þ cells in the skin is a characteristic finding in skin barrier defects, such as psoriasis and atopic dermatitis.. However, mechanistic insight into effects of IL-22 on epidermal functioning is yet to be elucidated.. One crucial step during epidermal differentiation is deimination or citrullination. Article Title: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. Article Snippet: List of Antibodies Used in the Study Antibody Application Working Dilution Source (Catalog Number) Anti-PAD1 (rabbit polyclonal) Western blot analysis 1:400 Abcam (ab181791) Anti-PAD2 (rabbit polyclonal) Western blot analysis, functional blocking assay 1:1,000 Proteintech (12110-1-AP) Anti-PAD3 (rabbit polyclonal) Western blot analysis 1:1,000 Novus (NBP1-92240) Anti-PAD1 (rabbit polyclonal) Immunofluorescence, immunohistochemistry 1:200 Sigma (HPA062294) PAD1 mAb (Clone 6B4) Functional blocking assay 1:1,000 Cayman Chemical (22997) PAD3 mAb (Clone 4E5) Functional blocking assay 1:1,000 Cayman Chemical (24377) Human IL-22 Ra1 antibody (polyclonal goat IgG) Receptor neutralization assay 1 mg/ml R&D Systems (AF2770-SP) Normal goat IgG control (polyclonal goat IgG) Receptor neutralization assay 1 mg/ml R&D Systems (Ab-108-C) Human IL-10Rb antibody (monoclonal mouse IgG1 clone #90220) Receptor neutralization assay 1 mg/ml R&D Systems (MAB874-SP) Mouse IgG1 isotype control (monoclonal mouse IgG1 k clone #11711) Receptor neutralization assay 1 mg/ml R&D Systems (MAB002) Anti-modified citrulline, clone C4 (mmAb) Western blot analysis 1:1,000 Merck Millipore (#MABS487) Anti-peptidyl-citrulline, clone F95 (mmAb) Immunofluorescence 1:200 Merck Millipore (#MABN328) AHF7 (FOVA711C) (mouse monoclonal) Western blot analysis 1:1,000 Simon et al., 1995 AHF3 (FOVA311C) (mouse monoclonal) Western blot analysis 1:1,000 Simon et al., 1995 Anti-deiminated keratin K1 (ACP) (rabbit polyclonal) Western blot analysis 1:1,000 Ishida-Yamamoto et al., 2002 Cytokeratin 1 (rabbit polyclonal) Western blot analysis 1:1,000 Proteintech (16848-1-AP-20) Beta actin mouse mAb Western blot analysis 1:10,000 Proteintech (66009-1-Ig) Goat anti-mouse IgM (heavy chain) antibody, Alexa Fluor 594 Immunofluorescence of F95 10 mg/ml Invitrogen (#A-21044) Goat anti-rabbit IgG (HþL) Alexa Fluor 488 Immunofluorescence of PAD1 1:100 JacksonimmunoResearch (111-545-003) Goat anti-rabbit IgG (HþL) HRP Western blot analysis 1:10,000 GeneTex (GTX213110-01) Mouse anti-IgG antibody (HþL) HRP Western blot analysis 1:10,000 GeneTex (GTX213112-01) Goat anti-rabbit IgG antibody (HþL), biotinylated Immunohistochemistry 1:400 Vector Laboratories (BA-1000- 1.5) Abbreviations: H, healthy control; HRP, horseradish peroxidase; L, lesional; PAD, peptidylarginine deiminase. Immunohistochemistry:Article Title: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. Article Snippet: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation Avinash Padhi, Ankit Srivastava, Abarajitha Ramesh, Marcus Ehrström, Michel Simon, Enikö Sonkoly, Liv Eidsmo, Peter Bergman and Josefin Lysell Increased presence of IL-22þ cells in the skin is a characteristic finding in skin barrier defects, such as psoriasis and atopic dermatitis.. However, mechanistic insight into effects of IL-22 on epidermal functioning is yet to be elucidated.. One crucial step during epidermal differentiation is deimination or citrullination. Article Title: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. Article Snippet: List of Antibodies Used in the Study Antibody Application Working Dilution Source (Catalog Number) Anti-PAD1 (rabbit polyclonal) Western blot analysis 1:400 Abcam (ab181791) Anti-PAD2 (rabbit polyclonal) Western blot analysis, functional blocking assay 1:1,000 Proteintech (12110-1-AP) Anti-PAD3 (rabbit polyclonal) Western blot analysis 1:1,000 Novus (NBP1-92240) Anti-PAD1 (rabbit polyclonal) Immunofluorescence, immunohistochemistry 1:200 Sigma (HPA062294) PAD1 mAb (Clone 6B4) Functional blocking assay 1:1,000 Cayman Chemical (22997) PAD3 mAb (Clone 4E5) Functional blocking assay 1:1,000 Cayman Chemical (24377) Human IL-22 Ra1 antibody (polyclonal goat IgG) Receptor neutralization assay 1 mg/ml R&D Systems (AF2770-SP) Normal goat IgG control (polyclonal goat IgG) Receptor neutralization assay 1 mg/ml R&D Systems (Ab-108-C) Human IL-10Rb antibody (monoclonal mouse IgG1 clone #90220) Receptor neutralization assay 1 mg/ml R&D Systems (MAB874-SP) Mouse IgG1 isotype control (monoclonal mouse IgG1 k clone #11711) Receptor neutralization assay 1 mg/ml R&D Systems (MAB002) Anti-modified citrulline, clone C4 (mmAb) Western blot analysis 1:1,000 Merck Millipore (#MABS487) Anti-peptidyl-citrulline, clone F95 (mmAb) Immunofluorescence 1:200 Merck Millipore (#MABN328) AHF7 (FOVA711C) (mouse monoclonal) Western blot analysis 1:1,000 Simon et al., 1995 AHF3 (FOVA311C) (mouse monoclonal) Western blot analysis 1:1,000 Simon et al., 1995 Anti-deiminated keratin K1 (ACP) (rabbit polyclonal) Western blot analysis 1:1,000 Ishida-Yamamoto et al., 2002 Cytokeratin 1 (rabbit polyclonal) Western blot analysis 1:1,000 Proteintech (16848-1-AP-20) Beta actin mouse mAb Western blot analysis 1:10,000 Proteintech (66009-1-Ig) Goat anti-mouse IgM (heavy chain) antibody, Alexa Fluor 594 Immunofluorescence of F95 10 mg/ml Invitrogen (#A-21044) Goat anti-rabbit IgG (HþL) Alexa Fluor 488 Immunofluorescence of PAD1 1:100 JacksonimmunoResearch (111-545-003) Goat anti-rabbit IgG (HþL) HRP Western blot analysis 1:10,000 GeneTex (GTX213110-01) Mouse anti-IgG antibody (HþL) HRP Western blot analysis 1:10,000 GeneTex (GTX213112-01) Goat anti-rabbit IgG antibody (HþL), biotinylated Immunohistochemistry 1:400 Vector Laboratories (BA-1000- 1.5) Abbreviations: H, healthy control; HRP, horseradish peroxidase; L, lesional; PAD, peptidylarginine deiminase. Neutralization:Article Title: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. Article Snippet: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation Avinash Padhi, Ankit Srivastava, Abarajitha Ramesh, Marcus Ehrström, Michel Simon, Enikö Sonkoly, Liv Eidsmo, Peter Bergman and Josefin Lysell Increased presence of IL-22þ cells in the skin is a characteristic finding in skin barrier defects, such as psoriasis and atopic dermatitis.. However, mechanistic insight into effects of IL-22 on epidermal functioning is yet to be elucidated.. One crucial step during epidermal differentiation is deimination or citrullination. Article Title: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. Article Snippet: List of Antibodies Used in the Study Antibody Application Working Dilution Source (Catalog Number) Anti-PAD1 (rabbit polyclonal) Western blot analysis 1:400 Abcam (ab181791) Anti-PAD2 (rabbit polyclonal) Western blot analysis, functional blocking assay 1:1,000 Proteintech (12110-1-AP) Anti-PAD3 (rabbit polyclonal) Western blot analysis 1:1,000 Novus (NBP1-92240) Anti-PAD1 (rabbit polyclonal) Immunofluorescence, immunohistochemistry 1:200 Sigma (HPA062294) PAD1 mAb (Clone 6B4) Functional blocking assay 1:1,000 Cayman Chemical (22997) PAD3 mAb (Clone 4E5) Functional blocking assay 1:1,000 Cayman Chemical (24377) Human IL-22 Ra1 antibody (polyclonal goat IgG) Receptor neutralization assay 1 mg/ml R&D Systems (AF2770-SP) Normal goat IgG control (polyclonal goat IgG) Receptor neutralization assay 1 mg/ml R&D Systems (Ab-108-C) Human IL-10Rb antibody (monoclonal mouse IgG1 clone #90220) Receptor neutralization assay 1 mg/ml R&D Systems (MAB874-SP) Mouse IgG1 isotype control (monoclonal mouse IgG1 k clone #11711) Receptor neutralization assay 1 mg/ml R&D Systems (MAB002) Anti-modified citrulline, clone C4 (mmAb) Western blot analysis 1:1,000 Merck Millipore (#MABS487) Anti-peptidyl-citrulline, clone F95 (mmAb) Immunofluorescence 1:200 Merck Millipore (#MABN328) AHF7 (FOVA711C) (mouse monoclonal) Western blot analysis 1:1,000 Simon et al., 1995 AHF3 (FOVA311C) (mouse monoclonal) Western blot analysis 1:1,000 Simon et al., 1995 Anti-deiminated keratin K1 (ACP) (rabbit polyclonal) Western blot analysis 1:1,000 Ishida-Yamamoto et al., 2002 Cytokeratin 1 (rabbit polyclonal) Western blot analysis 1:1,000 Proteintech (16848-1-AP-20) Beta actin mouse mAb Western blot analysis 1:10,000 Proteintech (66009-1-Ig) Goat anti-mouse IgM (heavy chain) antibody, Alexa Fluor 594 Immunofluorescence of F95 10 mg/ml Invitrogen (#A-21044) Goat anti-rabbit IgG (HþL) Alexa Fluor 488 Immunofluorescence of PAD1 1:100 JacksonimmunoResearch (111-545-003) Goat anti-rabbit IgG (HþL) HRP Western blot analysis 1:10,000 GeneTex (GTX213110-01) Mouse anti-IgG antibody (HþL) HRP Western blot analysis 1:10,000 GeneTex (GTX213112-01) Goat anti-rabbit IgG antibody (HþL), biotinylated Immunohistochemistry 1:400 Vector Laboratories (BA-1000- 1.5) Abbreviations: H, healthy control; HRP, horseradish peroxidase; L, lesional; PAD, peptidylarginine deiminase. Control:Article Title: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. Article Snippet: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation Avinash Padhi, Ankit Srivastava, Abarajitha Ramesh, Marcus Ehrström, Michel Simon, Enikö Sonkoly, Liv Eidsmo, Peter Bergman and Josefin Lysell Increased presence of IL-22þ cells in the skin is a characteristic finding in skin barrier defects, such as psoriasis and atopic dermatitis.. However, mechanistic insight into effects of IL-22 on epidermal functioning is yet to be elucidated.. One crucial step during epidermal differentiation is deimination or citrullination. Article Title: IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. Article Snippet: List of Antibodies Used in the Study Antibody Application Working Dilution Source (Catalog Number) Anti-PAD1 (rabbit polyclonal) Western blot analysis 1:400 Abcam (ab181791) Anti-PAD2 (rabbit polyclonal) Western blot analysis, functional blocking assay 1:1,000 Proteintech (12110-1-AP) Anti-PAD3 (rabbit polyclonal) Western blot analysis 1:1,000 Novus (NBP1-92240) Anti-PAD1 (rabbit polyclonal) Immunofluorescence, immunohistochemistry 1:200 Sigma (HPA062294) PAD1 mAb (Clone 6B4) Functional blocking assay 1:1,000 Cayman Chemical (22997) PAD3 mAb (Clone 4E5) Functional blocking assay 1:1,000 Cayman Chemical (24377) Human IL-22 Ra1 antibody (polyclonal goat IgG) Receptor neutralization assay 1 mg/ml R&D Systems (AF2770-SP) Normal goat IgG control (polyclonal goat IgG) Receptor neutralization assay 1 mg/ml R&D Systems (Ab-108-C) Human IL-10Rb antibody (monoclonal mouse IgG1 clone #90220) Receptor neutralization assay 1 mg/ml R&D Systems (MAB874-SP) Mouse IgG1 isotype control (monoclonal mouse IgG1 k clone #11711) Receptor neutralization assay 1 mg/ml R&D Systems (MAB002) Anti-modified citrulline, clone C4 (mmAb) Western blot analysis 1:1,000 Merck Millipore (#MABS487) Anti-peptidyl-citrulline, clone F95 (mmAb) Immunofluorescence 1:200 Merck Millipore (#MABN328) AHF7 (FOVA711C) (mouse monoclonal) Western blot analysis 1:1,000 Simon et al., 1995 AHF3 (FOVA311C) (mouse monoclonal) Western blot analysis 1:1,000 Simon et al., 1995 Anti-deiminated keratin K1 (ACP) (rabbit polyclonal) Western blot analysis 1:1,000 Ishida-Yamamoto et al., 2002 Cytokeratin 1 (rabbit polyclonal) Western blot analysis 1:1,000 Proteintech (16848-1-AP-20) Beta actin mouse mAb Western blot analysis 1:10,000 Proteintech (66009-1-Ig) Goat anti-mouse IgM (heavy chain) antibody, Alexa Fluor 594 Immunofluorescence of F95 10 mg/ml Invitrogen (#A-21044) Goat anti-rabbit IgG (HþL) Alexa Fluor 488 Immunofluorescence of PAD1 1:100 JacksonimmunoResearch (111-545-003) Goat anti-rabbit IgG (HþL) HRP Western blot analysis 1:10,000 GeneTex (GTX213110-01) Mouse anti-IgG antibody (HþL) HRP Western blot analysis 1:10,000 GeneTex (GTX213112-01) Goat anti-rabbit IgG antibody (HþL), biotinylated Immunohistochemistry 1:400 Vector Laboratories (BA-1000- 1.5) Abbreviations: H, healthy control; HRP, horseradish peroxidase; L, lesional; PAD, peptidylarginine deiminase. |